LABORATORY 6. MOLECULAR BIOLOGY

 

OVERVIEW

In this laboratory, you will investigate some basic principles of genetic engineering. Plasmids containing specific fragments of foreign DNA will be used to transform Escherichia coli cells, conferring antibiotic (ampicillin) resistance. Restriction enzyme digests of phage lambda DNA also will be used to demonstrate techniques for separating and identifying DNA fragments using gel electrophoresis.

OBJECTIVES

Section A: Before doing this laboratory you should understand:

Section B: After doing this laboratory you should be able to:

LAB GUIDE

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Lab 6- Electrophoresis